Nucleic Acid Extraction a i ʻole ka pahu hoʻomaʻemaʻe
Nucleic Acid Extraction Or Hoomaemae Kita i ʻole mālama ʻia ma -20 ℃. Pono e lawe ʻia ka laʻana me ka 0 ℃ curling.
Introduction
Hoʻolālā ʻia ka Nucleic Acid Extraction or Purification Kit (Magnetic Beads Method) no ka hoʻomaʻemaʻe automated o RNA a me DNA mai nā wai o ke kino (e like me ka swabs, plasma, serum) me ka hoʻohana ʻana i nā mea hana ʻokiʻoki nucleic acid. Hāʻawi ka ʻenehana magnetic-particle i ka DNA/RNA kiʻekiʻe i kūpono no ka hoʻohana pololei ʻana i nā noi o lalo e like me ka amplification a i ʻole nā hopena enzymatic ʻē aʻe.
Application Range
ʻO ke koko holoʻokoʻa, ka plasma, ka serum a me nā mea kikoʻī ʻē aʻe i hoʻokaʻawale pololei ʻia a ʻeli ʻia. Ua hoʻopili ʻia ka ʻakika nucleic i hoʻokuʻu ʻia e ka super paramagnetic nanometer magnetic beads. A laila ua hoʻoneʻe ʻia ka protein, nā ion paʻakai inorganic a me nā mea haumia organik e ka holoi ʻana i ka wai.
Kit Contents
| Cat. ʻAʻole. | YXN-VIRAL01-32A-BR | Nā ʻāpana | ||
| -50A | - 100A | |||
| Nui | 32Tes | 50 Hoao | 100 Hoao | |
| Lysis Buffer | 96 wellPre-pack ed Pāpā 2 ʻāpana | 25ml | 50ml | Surfactant a me Tris |
| Holoi Pahu I | ★15ml | ★30ml | Paʻakai paʻakai kiʻekiʻe | |
| Holoi Pahu Il | ★6ml*2 | ★12ml*2 | Haʻahaʻa paʻakai haʻahaʻa | |
| Elution Buffer | 10ml | 20ml | Haʻahaʻa paʻakai haʻahaʻa | |
| ʻO MagaBio Reagent | 1.0ml | 2.0ml | Mākēneki particles | |
| Puke lima(=YXN-VIRAL01-32A-BR) | 1 | 1 | 1 | |
| Notes:No ka meaYXN-VIRAL01-32A-BR-50A,e hoʻohui i 15mL Absolute ethanol i ★15mL Wash Buffer I ma mua o ka hoʻohana ʻana; e hoʻohui i 24mL Absolute ethanol i ka ★6mL Holoi pale Il ma mua o ka hoʻohana ʻana. | ||||
| No ka meaYXN-VIRAL01-32A-BR-100A, hoʻohui i 30mL Absolute ethanol i ★30mL Wash Buffer I ma mua o ka hoʻohana ʻana; e hoʻohui i 48mL Absolute ethanol i ★12mL Holoi paʻa Il ma mua o ka hoʻohana ʻana.【Reagents e hoʻomākaukau ʻia e ka mea hoʻohana】E ʻoluʻolu e hoʻomākaukau iā ʻoe iho i ka ethanol piha (analytical grade). | ||||
Stʻākala Conditions
I ka hōʻea ʻana mai o ka pahu, hiki ke mālama ʻia nā ʻāpana kit ma ke ana wela (15 - 25°C). Paʻa nā reagents a hiki i hoʻokahi makahiki mai ka lā hana.
Sample Requirements
1. Hoʻopili pili: Swabs, plasma, serum a me ke koko holoʻokoʻa etc.
2. Ka waiho ʻana o ka laʻana a me ka lawe ʻana: Pono e hoʻāʻo koke ʻia ka hāpana
Materials a Devices Required but Not Provided
1. Nā mīkina lima pauda ʻole
2. pahu Biohazard
3. Penikala a i ʻole
Procedure
Hoʻohana ka mea ma lalo iho nei i ka laʻana o ka mea hoʻoheheʻe hoʻoheheʻe ʻia ma ke ʻano he laʻana no ka wehewehe pōkole ʻana i nā ʻanuʻu hana o ka reagent unuhi ma luna o ka mea hoʻoheheʻe wai nucleic biological.Bioer NPA-32Pa i ʻoleSMART 32. No nā ʻano laʻana ʻē aʻe, e ʻoluʻolu e nānā i ka manual mea hoʻohana. Hiki ke hoʻohana ʻia e nā mea kūʻai aku e like me ka loaʻa hoʻokolohua:
1. Reagent Hoʻomākaukau
a. No ka meaYXN-VB03-32A-50A a YXN-VB03-32A-100A
Hoʻohui i ka 500uL Lysis Buffer i ke kolamu 1 a me 7 o ka pā 2.2mL 96-hohonu, 500uL Wash Buffer I i ke kolamu 2 a me 8, 500uL Wash Buffer II i ke kolamu 3, 4 a me 9,10; 70uL Elution Buffer i ke kolamu 5 a me 11, 180uL wai maʻemaʻe a me 20uL MagaBio Reagent i ke kolamu 6 a me 12 (pono e hui pono ʻia nā peʻa magnetic ma mua o ka hoʻohana ʻana),
b. No ka meaYXN-VB03-32A
E kau i ka 96 mau reagents i hookomo mua ia ma ka lumi wela. E lulu i ka pā 96-well ma luna i lalo no ʻekolu manawa, a hemo i ka ʻeke plastik. E hoʻopololei i ka reagent i hoʻopaʻa mua ʻia no kekahi mau kekona (a i ʻole e kowali me ka lima i kekahi mau manawa) e pale i ka reagent e pili ana i ka paia o nā paipu. Wehe i ke kiʻi ʻoniʻoni aluminika o ka pā 96-well a ʻike i ke kuhikuhi o ka pā (nā lei magnetic ma ke kolamu #6 a me #12),
2.Laʻana Ka unuhi
1. E hoʻohui i ka laʻana 300uL i nā kolamu pā luawai 96 #1 a me #7, e ʻoluʻolu e pale aku i ka hoʻohaumia ʻana,
2. E kau i ka papa punawai hohonu 96 i ka mea kani, e hookomo i ka 8-strip tips ma ka mea kani,
3. Holo i ka papahana e like me nā kaʻina hana aʻe,
4. Ma hope o ka pau ʻana o ka hoʻomaʻemaʻe ʻakomi, e hoʻololi i ka elution Buffer ma nā kolamu 5 a me 11 i kahi paipu centrifuge antinuclear maʻemaʻe 0.5mL; inā ʻaʻole hoʻohana koke ʻia, e ʻoluʻolu e mālama i ka-20 °C.
Performance Characteristics
1. ʻIke ʻia ka huahana i unuhi ʻia e ka HBV DNA detection reagent kiʻekiʻe e hiki ai i ka sensitivity o 10 IU/mL. ʻIke ʻia ka huahana i unuhi ʻia e ka HCV RNA detection reagent a hiki i ka 50 IU/mL.
2. E koho i 4 mau la'ana (serum/plasma sample, nasopharyngeal swab sample, cervical exfoliated cell sample), ua ho'ohehe'e 'ia kēlā me kēia la'ana i nā manawa he 10 me nā gradients 3 (me ka huina kumu kumu o 4 mau kumu), me ka ho'ohana 'ana i nā reagents kūpono a me nā mea ho'ā'o e 'ike i loko. gene reference e like me nā kuhikuhi huahana, a ʻokoʻa ka waiwai Ct o kēlā me kēia pūʻulu ma lalo o 1.
| ʻO Step | Pono Wahi | Program inoa | Waiting Time(min:SS) | Mixana Time(mi:SS) | Magnet Time(min:SS) | Adsorption | Spipi | Volume koko (μL) | Temperature |
| 1 | 1 | Lysis | 0:00 | 2:00 | 0:00 | F | 700 | 80 | |
| 2 | 6 | Beads | 0:00 | 0:15 | 0:15 | √ | F | 200 | |
| 3 | 1 | Bind | 0:00 | 3:00 | 0:45 | √ | F | 700 | |
| 4 | 2 | Wlehu1 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 5 | 3 | Wlehu2 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 6 | 4 | Wlehu3 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 7 | 5 | Elution | 2:00 | 2:30 | 0:30 | F | 70 | 80 | |
| 8 | 6 | E hoolei | 0:00 | 0:15 | 0:00 | F | 200 |
Safety
1. GENERAL PALAPALA.
ʻO ka hoʻohana ʻana i kēia huahana ma ke ʻano i hōʻike ʻole ʻia i loko o ka palapala mea hoʻohana e hopena i ka hōʻeha pilikino a i ʻole ka pōʻino i ka mea hana a i ʻole ka mea hana. E hōʻoia ua loaʻa i ka mea hoʻohana i kēia huahana nā ʻōlelo aʻo i nā hana palekana maʻamau no nā laboratories a me ka ʻike palekana i hāʻawi ʻia ma kēia palapala.
1.1 Ma mua o ka hoʻohana ʻana i kahi mea hana a mea hana paha, e heluhelu a hoʻomaopopo i ka ʻike palekana i hāʻawi ʻia i loko o ka palapala mea hoʻohana i hāʻawi ʻia e ka mea hana o ka mea hana a i ʻole ka mea hana.
1.2 Ma mua o ka lawelawe ʻana i nā mea kemika, heluhelu a hoʻomaopopo i nā Pepa ʻIkepili Palekana (SDS) kūpono a hoʻohana i nā lako pale pilikino kūpono (mau mīkina lima, ʻaʻahu, pale maka, etc.). No ka loaʻa ʻana o nā SDS, e ʻike i ka ʻāpana "Papa a me ke kākoʻo" ma kēia palapala.
2. Kemika palekana
HANA KIMIKA LAU. No ka hōʻemi ʻana i nā pōʻino, e hōʻoia i ka heluhelu ʻana a me ka hoʻomaʻamaʻa ʻana o ka poʻe limahana i nā alakaʻi palekana maʻamau no ka hoʻohana ʻana i ke kemika, ka mālama ʻana, a me ka ʻōpala i hāʻawi ʻia ma lalo nei, a e nīnau i ka SDS kūpono no ka mālama pono ʻana a me nā kuhikuhi kikoʻī: E heluhelu a hoʻomaopopo i ka Pepa ʻIkepili Palekana (SDSs) i hāʻawi ʻia e ka kemika. mea hana ma mua o kou mālama ʻana, mālama ʻana, a hana paha me nā kemika a i ʻole nā mea weliweli.
2.1 E ho'ēmi i ka pilina me nā kemika. E hoʻohana i nā lako pale pilikino kūpono i ka wā e lawelawe ai i nā mea kemika (e laʻa, nā makaaniani palekana, nā mīkina lima, a i ʻole nā lole pale).
2.2 E hoemi i ka inhalation o na kemika. Mai waiho hāmama nā ipu kemika. E hoʻohana wale me ka ea kūpono (no ka laʻana, fume hood).
2.3 E nānā maʻamau no ka lī ʻana a i ʻole nā kahe kemika. Inā hiki mai ka lika a i ʻole ke kahe ʻana, e hahai i nā kaʻina hana hoʻomaʻemaʻe a ka mea hana e like me ka mea i ʻōlelo ʻia ma ka SDS.
2.4 Hoʻohana i nā ʻōpala kemika i loko o kahi pahu hau.
2.5 E hōʻoia i ka hoʻohana ʻana i nā ipu ʻōpala mua a me ka lua. (He ʻōpala mua
paʻa ka pahu i ka ʻōpala koke. Aia i loko o ka pahu lua nā ʻōpala a i ʻole nā kahe mai ka ipu mua. Pono nā pahu ʻelua me nā mea ʻōpala a hoʻokō i nā koi federal, mokuʻāina, a me nā kūloko no ka mālama ʻana i nā ipu.).
2.6 Ma hope o ka hoʻokahe ʻana i kahi pahu ʻōpala, e hoʻopaʻa iā ia me ka pāpale i hāʻawi ʻia.
2.7 E hōʻike (ma ka nānā ʻana inā pono) i ka ʻōpala i hana ʻia e nā noi kūikawā, nā reagents, a me nā substrate i hoʻohana ʻia i kāu keʻena keʻena.
2.8 E hōʻoia i ka mālama ʻia ʻana o ka ʻōpala, hoʻoili ʻia, lawe ʻia, a hoʻolei ʻia e like me nā hoʻoponopono kūloko, mokuʻāina / panalāʻau, a / a i ʻole nā ʻāina.
2.9 Pono paha nā mea radioactive a i ʻole biohazardous i ka lawelawe kūikawā, a pili paha nā palena o ka hoʻokuʻu ʻana.
3.Koolaola pōʻino palekana
Hiki i Biohazard. Ma muli o nā laʻana i hoʻohana ʻia ma kēia mea hana, manaʻo ʻia ka ʻili he biohazard. E hoʻohana i nā ʻano hana decontamination kūpono i ka wā e hana ana me nā biohazard.
BIOHAZARD. ʻO nā laʻana olaola e like me nā ʻiʻo, nā wai kino, nā mea hoʻopili, a me ke koko o ke kanaka a me nā holoholona ʻē aʻe i hiki ke hoʻouna i nā maʻi maʻi. E hahai i nā hoʻoponopono kūloko, mokuʻāina / panalāʻau, a / a i ʻole aupuni. E hoʻohana i nā lako pale kūpono, e komo pū ana akā ʻaʻole i kaupalena ʻia: nā maka pale, pale maka, nā lole/ʻaʻahu lab, a me nā mīkina lima. Pono e hana ʻia nā hana a pau ma nā keʻena i hoʻolako pono ʻia me ka hoʻohana ʻana i nā lako palekana kūpono (e laʻana, nā mea hoʻopaʻa kino). Pono e hoʻomaʻamaʻa ʻia kēlā me kēia kanaka e like me nā koi kūpono a me nā ʻoihana / ʻoihana ma mua o ka hana ʻana me nā mea hiki ke maʻi.
Heluhelu a hahai i nā alakaʻi kūpono a/a i ʻole nā koi hoʻoponopono i kēia:
Ma US: Nā alakaʻi alakaʻi o ka US Department of Health and Human Services i paʻi ʻia ma Biosafety in Microbiological and Biomedical Laboratories loaʻa ma: www.cdc.gov/biosafety.
Nā Kūlana Palekana a me ke Ola Ola, Nā ʻAiʻi Koko (29 CFR§1910.1030), loaʻa ma:
www.access.gpo.gov/nara/cfr/waisidx_01/29cfr1910a_01.html
ʻO nā protocol Biosafety Program o kāu hui/keʻena no ka hana ʻana me/ka mālama ʻana i nā mea maʻi maʻi. Loaʻa ka ʻike hou aku e pili ana i nā alakaʻi biohazard ma: www.cdc.gov.
Ma EU: E nānā i nā alakaʻi kūloko a me nā kānāwai e pili ana i ka biohazard a me ka mālama ola olaola a e nānā i nā hana maikaʻi loa i paʻi ʻia ma ka World Health Organization (WHO) Laboratory Biosafety Manual, ʻekolu paʻi, loaʻa ma: www.who.int/csr/resources/publications /biosafety/WHO_CDS_CSR_LYO_200 4_11/en/.
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